Regulatory

Part:BBa_K4721000:Design

Designed by: Celeste Bos   Group: iGEM23_Leiden   (2023-10-08)


PfumC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

This part has been described by Schada Von Borzyskowski et al. (2015)[1]. The source of this part is the putative promoter region of the fumC gene, coding for a fumarase (MexAM1_METAp2857) in M. extorquens AM1

References

[1] Schada Von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. A set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in methylobacterium extorquens am1 and other alphaproteobacteria. ACS Synth Biol. 2015;4(4):430-443. doi:10.1021/sb500221v

[2] Peyraud R, Kiefer P, Christen P, Massou S, Portais JC, Vorholt JA. Demonstration of the ethylmalonyl-CoA pathway by using13C metabolomics. Proc Natl Acad Sci U S A. 2009;106(12):4846-4851. doi:10.1073/pnas.0810932106

[3] Leveau JHJ, Lindow SE. Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria. J Bacteriol. 2001;183(23):6752-6762. doi:10.1128/JB.183.23.6752-6762.2001